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. 2021 Feb 1;11:613079. doi: 10.3389/fimmu.2020.613079

Figure 4.

Figure 4

cGAS is essential for intracellular DNA-dependent IFN-I and MHC-II transcription in HD11 cells. (A) Example of identification of indel in clonally selected HD11 cGAS KO using NGS sequencing. (B) WT and cGAS KO HD11 cells were transfected with HT-DNA, CT-DNA (2 μg/ml) or Poly(I:C) (1 μg/ml) for 6 h and transcription of the indicated genes measured by qRT-PCR. (C) cGAS KO HD11 cells were primed with IFNα for 6 h, transfected with HT-DNA, CT-DNA, or Poly(I:C) and transcription of IFNB and ISG12.2 measured by qRT-PCR 6 h later (D) WT or cGAS KO cells were treated with 2’3’cGAMP (10 μg/ml) and transcription of IFNB measured by qRT-PCR 6 h later. *p < 0.05, **p < 0.01, ***p < 0.001; ns, no significant difference. Data is representative of two or more replicates.