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. 2021 Feb 1;11:613079. doi: 10.3389/fimmu.2020.613079

Figure 6.

Figure 6

Fowlpox triggers a cGAS/STING dependent DNA sensing pathway in HD11 cells. (A) HD11 cells were infected with FWPV strain FP9 at a multiplicity of infection of three. Twenty-four hours later, RNA was extracted and qRT-PCR carried out for the indicated genes. (B, C) HD11 WT, cGAS or STING KO cells were infected with FP9 (black bars), FPV012 (blue bars), FPV184 (red bars), at a multiplicity of infection of three. Twenty-four hours later, RNA was extracted and qRT-PCR carried out for the indicated genes. (D) HD11 cells were infected with FWPV strain FP9 at a multiplicity of infection of three. Twenty-four hours later, RNA was extracted and qRT-PCR carried out for the indicated FWPV genes. (E) HD11 WT, cGAS or STING KO cells were infected with FP9, FPV012, or FPV184 at a multiplicity of infection of three, cell supernatants harvested and released FP9 was titrated on CEFs to measure focus forming units (ffu) per ml. *p < 0.05, **p < 0.01, ***p < 0.001; ****p < 0.0001; ns, no significant difference. Data is representative of two or more replicates.