Figure 2.

Proliferation of Qinchuan bovine pre-adipocytes is promoted by bta-miR-150. (A,B) Bovine adipocytes transfected with mimic, mimic NC, inhibitor, and inhibitor NC. Cells harvested at 48 h after transfection were stained red with EdU (to indicate DNA replication) and blue with Hoechst (cell nuclei) (C) CCK-8 assays of bovine adipocytes. After incubating with 10% CCK-8 for 4 h, absorbance was measured at a wavelength of 450 nm. (D1–D3) Changes in mRNA levels of proliferation-related target genes according to qPCR findings. (E, F1–F3) Proliferation-related protein quantitation according to western blot findings. Error bars represent mean ± SD. N = 3 replicates. *Significantly different; *p < 0.05, **p < 0.01, ***p < 0.001, Student t-tests.