Table 2.
mRNA and miRNA Real-time quantitative PCR primer sequences.
| Genes | Primer Sequence (5′-3′) | Annealing |
|---|---|---|
| temperature | ||
| β-actin | F: CATCGGCAATGAGCGGTTCC R: CCGTGTTGGCGTAGAGGTC |
60°C |
| PCNA | F: CCTTGGTGCAGCTAACCCTT R: TTGGACATGCTGGTGAGGTT |
60°C |
| CDK1 | F:AGTGGAAACCAGGAAGCTTAG R:ATTCGTTTGGCAGGATCATAGA |
60°C |
| CDK2 | F:GGGTCCCTGTTCGTACTTATAC R:CCACTGCTGTGGAGTAGTATTT |
60°C |
| AKT-1 | F:AAGGAGATCATGCAGCACCGATTC R: GTCTTGGTCAGGTGGCGTAATGG |
60°C |
| CEBPα | F: ATCTGCGAACACGAGACG R: CCAGGAACTCGTCGTTGAA |
60°C |
| PPARγ | F: GACGACAGACAAATCACCGT R: CTTCCACGGAGCGAAACTGA |
60°C |
| FABP4 | F:TGAGATTTCCTTCAAATTGGG R: CTTGTACCAGAGCACCTTCATC |
60°C |
| RRAGD | F: TAAGAAGGAGCGGCAAGT R: GGAAGTCCCAAATCTGAAA |
60°C |
| RPS6KA3 | F:ACCTAGCAACATTCTTTATGTGGAT R: GCATCATAGCCTTGTCGT |
60°C |
| RPS6KB1 | F: ATCACCAAGGTCACGTCAAAC R: TGCTCCCAAACTCCACCAAT |
60°C |
| LPIN1 | F: AGTGAATCTTCAGATGCGTTTA R: CAGGTGTCGGCTTCGTTT |
60°C |
| NFKB1 | F: GAGAACTTCGAGCCTCTGTAC R: CTCATAGGGTTTCCCATTTA |
60°C |
| EIF4E | F: TCTAATCAGGAGGTTGCT R: CCCACATAGGCTCAATAC |
60°C |
| PIK3CA | F:TGGGTTTCTCGGTCCTAA R:CAGTCCGTCCAGTCATCC |
60°C |
| TNFRSF1B | F: CCAGCACAGCTCCAAGCA R:CAACTATCAGAAGCAGACCCAAT |
60°C |
| INSR | F: GGAAGGCGAGAAGACCAT R: TGACACCAGGGCATAGGA |
60°C |
| EIF4EBP2 | F: GTTCCTGATGGAGTGTCGGA R: AACTGTGACTCTTCACCGCCT |
60°C |
| PIK3R1 | F: GAGCGGGAAGAGGACATTGA R: TCTCCCCTGTCGTCTCGTTA |
60°C |
| mTOR | F: TGATGCAGAAGGTCGAGGTG R: GATGGGTGTCTATCGCCCAG |
60°C |
| PIK3R1 | F: CTATCTCCTGGACTTACCG R: GGCGACCTAATGAGTTTC |
60°C |
| U6 | F:TAGCCACCCTCAAGTATGTTCG R:CGAGGTAGGAGGACAGGAGT |
60°C |
| miR-150 | UCUCCCAACCCUUGUACCAGUGU |
60°C |