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. 2021 Jan 28;8:602707. doi: 10.3389/fcell.2020.602707

FIGURE 5.

FIGURE 5

circPDHX negatively regulated miR-378a-3p expression in prostate cancer (PCa) cells. (A) Schematic representation of the binding sites of miR-378a-3p with WT or Mut circPDHX 3′ untranslated region (UTR). (B,C) Assessment of luciferase activities of WT or Mut circPDHX 3′UTR after cotransfection with miR-378a-3p mimic or inhibitor and WT or Mut circPDHX 3′UTR reporter vectors in 22RV1 and PC3 cells. (D) Quantitative real-time PCR (qRT-PCR) analysis of the expression levels of miR-378a-3p after transfection with miR-378a-3p inhibitor or mimic in 22RV1 and PC3 cell lines. (E) qRT-PCR analysis of the expression levels of miR-378a-3p after transfection with circPDHX plasmids or si-circPDHX in 22RV1 and PC3 cell lines. (F,G) RNA immunoprecipitation (RIP) analysis of the enrichment levels of circPDHX and miR-378a-3p pulled down from Ago2 protein in 22RV1 and PC3 cells. (H,I) 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) analysis of cell proliferation after cotransfection with circPDHX plasmids and miR-378a-3p mimic or si-circPDHX and miR-378a-3p inhibitor in 22RV1 and PC3 cells. Data shown are the mean ± SEM of three experiments. *P < 0.05, **P < 0.01.