Figure 2.

KLF2 directly binds to the P1 promoter. The binding of KLF2 to the P1 promoter was determined by ChIP-qPCR. DF1 cells were transfected with a pCMV-Myc-KLF2 or pCMV-Myc empty construct. At 48 h after transfection, ChIP-qPCR was performed using a Myc-specific antibody or mouse normal IgG. Nonimmunoprecipitated DNA (2%) was used as an input chromatin. Data were normalized to the negative control, which was provided by the cotransfection of DF1 cells with pCMV-Myc and immunoprecipitation with mouse IgG. All data represent the mean ±SEM. Statistical analysis was determined using Student's t-test, **p < 0.01.