Figure 4.

KLF2 overexpression alters the histone modifications H3K9me2 and H3K27ac in the P1 promoter. ICP1 cells were transfected with pCMV-Myc-KLF2 or pCMV-Myc empty vector. At 48 h after transfection, the cells were harvested and subjected to ChIP-qPCR to evaluate the enrichment of H3K9me2 and H3K27ac in the P1 promoter using H3K9me2 and H3K27ac antibodies, respectively. Mouse and rabbit normal IgG were used as a negative control. Nonimmunoprecipitated DNA (2%) was used as an input chromatin. Data were normalized to the negative control, which was provided by the cotransfection of cells with empty pCMV-Myc vector and immunoprecipitation with normal IgG. All data represent the mean ± SEM. Statistical significance was determined using Student's t-test. **p < 0.01.