Figure 5.

KLF2 overexpression inhibits the endogenous expression of PPARγ1. (A,B) qRT-PCR analysis showing the effect of KLF2 overexpression on endogenous PPARγ1 expression in DF1 and ICP1 cells. Cells were transfected with pCMV-Myc-KLF2 or pCMV-Myc empty vector. At 48 h after transfection, the relative expression level of PPARγ1 was detected by qRT-PCR. Chicken NONO was used as an internal control. (C,D) Western blot analysis showing the effect of KLF2 overexpression on the endogenous PPARγ protein expression in DF1 and ICP1 cells. The cells were transfected with either pCMV-Myc-KLF2 or pCMV-Myc empty vector. The cell lysates were harvested, and the expression of PPARγ, KLF2, and β-actin protein was detected by Western blotting using anti-PPARγ (PPARγ), anti-Myc (Myc), and anti-β-actin (β-actin). β-actin was used as a loading control. Quantification of the relative PPARγ protein levels (expressed as the percentage of the cells transfected with pCMV-Myc empty vector, set as 1.0) was performed by analyzing western blot data using the Image J software. Data are means ± SEM, *p < 0.05, **p < 0.01.