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. 2021 Feb 9;8:610773. doi: 10.3389/fcell.2020.610773

Figure 3.

Figure 3

Maternal UHRF1 deficiency affects the H3K4me3 expression pattern but not H3K9me3 in zygote and two-cell embryos. (A) Immunofluorescent-staining using antibodies against H3K4me3 (in green) and UHRF1 (in red) at GV oocytes, zygotes, and two-cell embryos from control and Gdf9-cKO mice are shown. DNA was counterstained with DAPI (blue). Scale bar = 50 μm. (B) Quantification of the relative expression levels of H3K4me3 in control and Gdf9-cKO GV oocytes, zygotes, and two-cell embryos of (A). Data are presented as mean ± SEM. n = 3 independent experiments (total number of GV oocytes analyzed: n = 80 for control, n = 75 for Gdf9-cKO; total number of zygotes analyzed: n = 123 for control, n = 106 for Gdf9-cKO; total number of two-cell embryos analyzed: n = 96 for control, n = 83 for Gdf9-cKO), *p < 0.05. (C) Immunofluorescent-staining using antibodies against H3K9me3 (in green) and UHRF1 (in red) at GV oocytes, zygotes, and two-cell embryos from control and Gdf9-cKO mice are shown. DNA was counterstained with DAPI (blue). Scale bar = 50 μm. (D) Quantification of the relative expression levels of H3K9me3 in control and Gdf9-cKO GV oocytes (total number analyzed: n = 89 for control, n = 92 for Gdf9-cKO), zygotes (total number analyzed: n = 135 for control, n = 131 for Gdf9-cKO), and two-cell embryos (total number analyzed: n = 125 for control, n = 93 for Gdf9-cKO) of (C).