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. 2021 Feb 9;8:610773. doi: 10.3389/fcell.2020.610773

Figure 4.

Figure 4

Abnormal ZGA upon absence of maternal UHRF1 revealed by transcriptome analysis. (A,B) Volcano plots showing the RNA-seq data obtained from (A) MII oocytes and (B) two-cell embryos of control and Gdf9-cKO mice. Red and dark blue dots indicate genes that were significantly upregulated and downregulated, respectively (fold change > 2, and FDR < 0.05) in Gdf9-cKO oocytes or embryos. (C,D) RNA-seq data from (C) MII oocytes or (D) two-cell embryos in comparison with the different categories of the gene catalog available at the Database of Transcriptome in Mouse Early Embryos (DBTMEE) generated the transcriptome analysis. The total number of genes in each class and found in this study is indicated on top of the graph. (E) RT-qPCR validates the expression of select upregulated and downregulated genes in MII oocytes and two-cell embryos from control and Gdf9-cKO mice. *p < 0.05 by Student's t test. (F,G) Top 10 representative GO terms (biological functions) enriched in Gdf9-cKO (F) MII oocytes and (G) two-cell embryos. p value indicates the significance of the enrichment.