Skip to main content
. 2021 Feb 9;8:610773. doi: 10.3389/fcell.2020.610773

Figure 5.

Figure 5

Increased retrotransposons and γ-H2AX in MII oocytes and two-cell embryos depleted for maternal UHRF1. (A,B) Volcano plots depicting activation of individual retrotransposon repetitive elements at (A) MII oocytes and (B) two-cell embryos of Gdf9-cKO mice. Red and dark blue dots indicate repeat classes with significantly increased and decreased expression, respectively, in Gdf9-cKO compared with those in controls. (C,D) Pie chart representing the percentage of each category of repeat elements analyzed in (C) MII oocytes and (D) two-cell embryos RNA-seq data. (E,F) The representative immunofluorescent staining images for anti-LINE1 Orf1 at (E) MII oocytes and (F) two-cell embryos from control and Gdf9-cKO mice are shown. Nuclei were stained with DAPI. Scale bar = 50 μm. (G,H) Immunostaining images using antibodies directed against γ-H2AX (a marker of DNA strand break) for (G) MII oocytes and (H) two-cell embryos from control and Gdf9-cKO are shown. Nuclei were stained with DAPI. Scale bar = 50 μm.