Figure 1.

Generation and characterisation of rat embryonic stem cell derived macrophages. (A) Schematic diagram of rat embryonic stem cell (ESC)-derived macrophage differentiation. Confluent rat ESC are shown at day 0 (Clone DA5.2), embryoid bodies at day 7, and ESC-derived macrophages (ESDM) at day 25. Bars = 50, 100, and 50 µm, respectively. Images are representative of three repeat experiments, two replicates. (B) ESDM were cultured with or without (control) fluorescein labelled Zymosan A BioParticles. Images are representative of three repeat experiments, two replicates. Blue = nuclear DAPI staining. Bars = 10 µm. (C) Flow cytometry of permeabilized cells was used to assess purity of ESDM via CD68 expression. Quadrants were set using an isotype control. Dot plot is representative of three repeat experiments, two replicates.