Skip to main content
. 2021 Feb 1;11:594594. doi: 10.3389/fimmu.2020.594594

Figure 2.

Figure 2

Adult rat macrophage populations. (A) Macrophages were isolated from adult male Dark Agouti rats. Images are representative of cells isolated from 10 rats. Bars = 20 µm (AM) or 50 µm (BMDM, MDM, and PM). (B) Permeabilized macrophages were analysed by flow cytometry to assess purity via CD68 expression. Quadrants were set with isotype controls. Dot plots are representative of cells isolated from three rats. (C) Alveolar macrophages were cultured with or without (control) fluorescein labelled Zymosan A BioParticles and imaged with a Zeiss AxioVert. Images are representative of cells isolated from three rats. Bar = 50 µm. (D) Macrophages were cultured with or without (control) fluorescein labelled Zymosan A BioParticles and imaged with a Zeiss LSM 710 confocal. Images are representative of cells isolated from three rats. Bar = 10 µm. AM = alveolar macrophages, BMDM = bone marrow derived macrophages, MDM = monocyte derived macrophages, PM, peritoneal macrophages.