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. 2021 Feb 1;11:594594. doi: 10.3389/fimmu.2020.594594

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Copyright © 2021 Pridans, Irvine, Davis, Lefevre, Bush and Hume

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of rat macrophage gene expression in vitro. Microarray analysis was performed on in vitro cultured rat macrophages. (A) Hierarchical clustering of the top 5,000 differentially expressed genes sorted by false discovery rate (FDR; <0.009418). Three biological replicates were used for alveolar (AM), bone marrow-derived (BMDM), monocyte-derived (MDM) and peritoneal macrophages (PM), including technical replicates. Technical replicates were used ESC-derived macrophages (ESDM) obtained from a single ESC clone (DA5.2). (B) Graph shows the numbers of differential expressed genes for each of the rat macrophages. (C) Gene ontology-based gene sets that were enriched in each macrophage population compared to all the others were identified using Gene Set Enrichment Analysis.