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. 2021 Jan 8;18:1–12. doi: 10.2142/biophysico.bppb-v18.001

Figure 4 .

Figure 4 

SOS and RAF translocation and effects of a MEK inhibitor. (A) Membrane translocation time courses of GFP-SOS (left) and GFP-RAF (right) in cells co-expressing Halo-SHC (green) or Halo-SHC3F (orange), or with an SHC-knockdown (blue). (B, C) Membrane translocation time courses of GFP-GRB2, Halo-SHC, GFP-SOS, and GFP-RAF in cells pretreated with 10 μM U0126. GRB2, SOS, and RAF were co-expressed with Halo-SHC (green) or Halo-SHC3F (orange). (D) Membrane translocation time courses of SOS R1131K-Halo in cells with co-expression of WT-SHC (green) and SHC3F (orange). Vertical axes were normalized to the values obtained before stimulation. The mean values for 8–40 (A) or 13–30 (B, C) or 8–16 (D) cells were plotted along with the standard errors.