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Schematic of CRISPR-SE: (A) CRISPR-SE scans all of the 20-nucleotides (20-mer) gRNAs; (B) sort all gRNAs by the proximal region (10-nt close to PAM sequence); (C) Group gRNAs with the same proximal regions. (D) Calculate the mismatches in the proximal region; add the mismatches in the distal region only with similar proximal region. Using this approach, a query gRNA are virtually compared with all gRNAs.
