Table 1.

Many CRISPR design methods require a search engine to retrieve list of off-target sites. Newer methods are likely to use accurate brute force approaches

Year	Method	Search Engine	Mismatches	URL
2016	PhytoCRISP-Ex (35)	BLAST	2	http://www.phytocrispex.biologie.ens.fr/CRISP-Ex/
2013	CRISPRTarget (36)	BLAST	1	http://bioanalysis.otago.ac.nz/CRISPRTarget
2014	CRISPR-P (37)	BLAST	4	http://cbi.hzau.edu.cn/crispr/
2014	CRISPR-GA (38)	BLAT	1	http://crispr-ga.net
2014	CRISPR-ERA (22)	Bowtie	2	http://CRISPR-ERA.stanford.edu
2016	CHOPCHOP (23)	Bowtie	2	https://chopchop.cbu.uib.no/
2014	CasFinder (24)	Bowtie	2	http://arep.med.harvard.edu/CasFinder/
2015	CCTop (25)	Bowtie	4	http://crispr.cos.uni-heidelberg.de/
2014	GT-Scan (26)	Bowtie	3	http://gt-scan.braembl.org.au/gt-scan/
2016	CLD (27)	Bowtie	3	https://github.com/boutroslab/cld
2014	E-CRISP (28)	Bowtie	3	http://www.e-crisp.org/E-CRISP/
2018	CRISPR-RT (29)	Bowtie2	3	http://bioinfolab.miamioh.edu/CRISPR-RT/
2016	CT-Finder (30)	Bowtie2	4	http://bioinfolab.miamioh.edu/ct-finder/
2016	BreakingCas (31)	BWA	4	http://bioinfogp.cnb.csic.es/tools/breakingcas
2013	CRISPR-DO (32)	BWA	3	http://cistrome.org/crispr/
2016	CRISPOR (33)	BWA	4	http://crispor.tefor.net/
2017	CRISPETa (34)	BWA	2	http://crispeta.crg.eu/
2014	CRISPy (18)	K-mer	3	http://staff.biosustain.dtu.dk/laeb/crispy/
2015	off-spotter (19)	K-mer	5	https://cm.jefferson.edu/Off-Spotter/
2014	CRISPRdirect (20)	K-mer	5	http://crispr.dbcls.jp/
2017	GuideScan (12)	Brute force	2	https://bitbucket.org/arp2012/guidescan_public
2014	Cas-OffFinder (54)	Brute force	Any	http://www.rgenome.net/cas-offinder/
2018	FlashFry (55)	Brute force	5	https://github.com/aaronmck/FlashFry
2019	Crisflash (56)	Brute force	4	https://github.com/crisflash
2019	CRISPRitz (21)	Brute force	Any	https://github.com/pinellolab/CRISPRitz
2020	Crackling	Brute force	4	https://github.com/bmds-lab/Crackling
2020	CRISPR-SE	BruceForce	Any	http://renlab.sdsc.edu/CRISPR-SE/