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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: J Leukoc Biol. 2020 Sep 17;109(1):161–172. doi: 10.1002/JLB.2MA0820-259R

Figure 2. Role of CARD9 signaling in dendritic cell responses to Malassezia.

Figure 2.

(A) BMDC from wild type and the indicated knockout mice were treated with live M. restricta, M. globosa, or C. albicans at MOI=5 or with 100 ng/ml LPS. Supernatants were collected after 24 hr, and production of the indicated cytokines was measured by LegendPlex. (B) BMDC from wild type and the indicated knockout mice were incubated for the indicated periods of time with eFluor 670 fluorescently labeled yeasts, and phagocytosis was quantified by flow cytometry. (C) BMDC from wild type and the indicated knockout mice were treated with live M. restricta or M. globosa (MOI=5) or not (UT = untreated), and production of reactive oxygen species (ROS) was measured by luminol-enhanced chemiluminescence (RUL=relative light units). The experiments in this figure are representative of two independent experiments. All data represent measurements done in triplicate ± SD, and statistics were done by student t-test * p≤0.05, ** p≤0.01,*** p≤0.001.