Figure 4:

Ultrastructural analysis of GFP-positive elements in the rat basal ganglia, after intra-striatal injection of rAAV2-retro-hSyn-Jaws-GFP. A-D: Representative electron micrographs of GFP-immunopositive elements (revealed with peroxidase). (A) GFP-labeled axonal terminal in striatum contralateral to the injection site, (B) labeled terminals in GPe ipsilateral to the injection site, (C-D) labeled terminal and dendrites in ipsilateral STN. Abbreviations: t (axon terminal), d (dendrite), sp (spine). White or black text for these abbreviations indicate if the element is GFP-positive or negative, respectively. Arrowhead denotes asymmetric synapse made by labeled terminals. All scale bars: 1μm. (E) Quantification of diameter (top) and number of visible mitochondria inside immunolabeled terminals (bottom) in the contralateral striatum (left) and ipsilateral GPe (right). Numbers in bars refer to total terminal counts for each category.