Figure 5:

Ultrastructural analysis of GFP-positive elements in the monkey basal ganglia after intra-striatal injection of rAAV2-retro-hSyn-Jaws-GFP. A-D: Representative electron micrographs of GFP-immunopositive elements (revealed with peroxidase). (A) GFP-positive terminal in putamen ipsilateral to injection site, (B) labeled terminal in putamen contralateral to injection site, (C) labeled dendrite in ipsilateral GPe, (D) labeled dendrite in ipsilateral STN. Abbreviations: t (axon terminal), d (dendrite), sp (spine). White or black text for these abbreviations indicate if the element is GFP-positive or negative, respectively. Arrowhead denotes asymmetric synapse made by labeled terminals. All scale bars: 1μm. (E) Quantification of diameter (top) and number of visible mitochondria inside immunolabeled terminals (bottom) in the ipsilateral (left) and contralateral (right) putamen. Numbers in bars refer to total terminal counts for each category.