TABLE 3.
Distribution of hemolysis measurements, hemoglobin, and ferritin by blood donor BMI and sex
| Sex | BMI (kg/m2) | <18.5 | 18.5-24.9 | 25-29.9 | 30-34.9 | 35-39.9 | 40-44.9 | 45-49.9 | ≥50 |
P (ANOVA)c |
|---|---|---|---|---|---|---|---|---|---|---|
| Males | Osmotic hemolysis (%)a | 27.5 ± 10.5 (N = 28) | 28.5 ± 13.1 (N = 1724) | 30.8 ± 13.3 (N = 2765) | 33.2 ± 14.0 (N = 1248) | 32.1 ± 14.2 (N = 413) | 32.9 ± 14.1 (N = 129) | 31.9 ± 14.5 (N = 32) | 31.2 ± 15.9 (N = 21) | <0.001 |
| Oxidative hemolysis (%)a | 38.6 ± 9.7 (N = 24) | 35.3 ± 10.4 (N = 1387) | 37.0 ± 9.8 (N = 2242) | 39.5 ± 9.7 (N = 1072) | 40.9 ± 10.0 (N = 356) | 40.8 ± 7.8 (N = 110) | 44.0 ± 10.4 (N = 28) | 41.7 ± 8.4 (N = 20) | <0.001 | |
| Hemoglobin (g/dL)a | 14.8 ± 1.0 (N = 29) | 14.7 ± 1.1 (N = 1757) | 14.7 ± 1.1 (N = 2809) | 14.6 ± 1.1 (N = 1248) | 14.7 ± 1.2 (N = 416) | 14.5 ± 1.0 (N = 134) | 14.4 ± 1.0 (N = 30) | 13.8 ± 1.0 (N = 21) | 0.002 | |
| Storage hemolysis (%)b | 0.33 ± 0.16 (N = 28) | 0.32 ± 0.18 (N = 1717) | 0.34 ± 0.20 (N = 2750) | 0.35 ± 0.21 (N = 1240) | 0.35 ± 0.22 (N = 411) | 0.32 ± 0.19 (N = 129) | 0.36 ± 0.12 (N = 32) | 0.44 ± 0.33 (N = 21) | <0.001 | |
| Ferritin (ng/mL)b | 32 ± 32 (N = 29) | 42 ± 60 (N = 1796) | 43 ± 68 (N = 2877) | 45 ± 72 (N = 1278) | 52.5 ± 78 (N = 426) | 50 ± 84.5 (N = 135) | 69.5 ± 94.25 (N = 32) | 80 ± 80 (N = 21) | <0.001 | |
| Females | Osmotic hemolysis (%)a | 24.6 ± 11.6 (N= 36) | 24.5 ± 12.1 (N = 2530) | 26.0 ± 12.1 (N = 1925) | 27.8 ± 12.5 (N = 1085) | 28.5 ± 13.0 (N = 484) | 29.6 ± 12.9 (N = 189) | 28.1 ± 13.1 (N = 76) | 32.6 ± 11.8 (N = 32) | <0.001 |
| Oxidative hemolysis (%)a | 33.1 ± 11.5 (N = 26) | 34.1 ± 9.6 (N = 1983) | 36.2 ± 9.6 (N = 1581) | 37.5 ± 9.3 (N = 893) | 39.2 ± 9.9 (N = 419) | 40.7 ± 10.2 (N = 160) | 39.2 ± 9.7 (N = 65) | 41.9 ± 7.7 (N = 28) | <0.001 | |
| Hemoglobin (g/dL)a | 13.2 ± 0.8 (N = 37) | 13.2 ± 0.9 (N = 2614) | 13.3 ± 0.9 (N = 1993) | 13.3 ± 1.1 (N = 1093) | 13.2 ± 0.9 (N = 496) | 13.2 ± 1.0 (N = 196) | 13.1 ± 0.9 (N = 77) | 13.2 ± 1.1 (N = 30) | 0.151 | |
| Storage hemolysis (%)b | 0.29 ± 0.09 (N = 35) | 0.29 ± 0.16 (N = 2522) | 0.30 ± 0.17 (N = 1922) | 0.30 ± 0.17 (N = 1084) | 0.30 ± 0.17 (N = 483) | 0.32 ± 0.17 (N = 189) | 0.32 ± 0.18 (N = 76) | 0.31 ± 0.19 (N = 32) | <0.001 | |
| Ferritin (ng/mL)b | 23 ± 21.8 (N = 38) | 21.5 ± 30 (N = 2658) | 23 ± 35 (N = 2015) | 25 ± 34 (N = 1121) | 26 ± 35 (N = 502) | 33.5 ± 37 (N = 198) | 35 ± 39.5 (N = 79) | 31 ± 30.5 (N = 32) | <0.001 |
Note: Data derived from 13 317 blood donors who participated in the National Heart, Lung, and Blood Institute Red Blood Cell Omics (RBC-Omics) study between 2013 and 2015. The total number of observations is noted by N. Sample numbers may differ among variables, as some data were not available for all subjects in the RBC-Omics database.
Abbreviations: ANOVA, analysis of variance; BMI, body mass index.
Variables with normal distribution reported as mean ± SD.
Variables that are not normally distributed and reported as median ± interquartile.
The P values were derived from ANOVA tests across all BMI groups, except for storage hemolysis and ferritin (not normally distributed), in which the P values derived from nonparametric Kruskal-Wallis test. We considered P values <0.001 as the statistically significant threshold to account for multiple statistical tests.