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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Matrix Biol. 2020 Nov 4;96:87–103. doi: 10.1016/j.matbio.2020.10.006

Figure 7. α5 Integrin Activation Requirement is Different for Matrix Remodeling Versus Inflammation.

Figure 7.

A/B) Talin1 L325R MLECs were plated on basement membrane proteins (diluted Matrigel) for 4 hours and treated with either oxLDL (100 μg/ml), α5 CHAMP (4 μM), or both for 24 hours. Fibronectin in the DOC-insoluble matrix was visualized by immunocytochemistry, and (B) quantified for mean fluorescent intensity. Representative images are shown. n=4–7. Scale bar = 25 μm. C. Talin1 L325R MLECs were plated on fibronectin and treated with either oxLDL, α5 CHAMP, or both for 1 hour. NF-κB activation was assessed by Western blotting for NF-κB phosphorylation (P-NF-κB (p65, Ser536)). Representative blots are shown. n=4. Values are means ±SE. *p<0.05, **p<0.01, ***p<0.001. Two-way ANOVA with Bonferroni posttest was used for statistical analyses.