Figure 1:

Siah2 co-localizes with PDGFRα⁺ cells and macrophages (IBA1). Representative images of paraffin embedded inguinal (iWAT) and epididymal (eWAT) adipose tissue sections from male mice on a 60% high fat diet (HFD) or 10% low fat diet (LFD) stained for nuclei (DAPI, blue), adipocyte precursor cells (PDGFRα, red), and macrophage (IBA1,green). Siah2 mRNA (gray) was localized by in situ hybridization using ACD RNAscope technology. The tissue was separately stained for perilipin 1 (PLIN1, yellow) and imaged at the same area to provide details on the structure of surrounding mature adipocytes. Signal brightness of everything except Siah2 was dimmed in the merged image to enhance Siah2 visualization. The arrows are added to emphasize Siah2 location. Data represent observations from N=8/group.