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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Obesity (Silver Spring). 2020 Nov 6;29(1):98–107. doi: 10.1002/oby.23013

Figure 4:

Figure 4:

SIAH2 affects Ebf1 and Zfp521 mRNA and protein expression during adipogenesis of adipose tissue primary stromal cells. (A) Pparg, Adiponectin (AdipoQ), Lpl, aP2 and (B) Siah2, Ebf1, Zfp423 and Zfp521 expression between wild-type and Siah2 knockout (Siah2KO) by qRT-PCR at the indicated time points. (C) Western blot analysis of EBF1, ZFP521 and SIAH2 expression in wild-type and Siah2KO at early time points post induction of adipogenesis. β-actin is included as a loading control. Bar graph plotted as mean ± SD; *, p < 0.0001; #, p < 0.001. Each data point in the bar graph represents technical replicates from a pooled sample (N=8–10 mice). The experiment was performed twice independently.