Figure 2.

Emergent technologies to investigate kinase pathways. (A) FRET-based kinase sensors are comprised of kinase specific phosphopeptide sequence and a phosphopeptide binding motif. (B) Analog-sensitive kinases are an important tool for direct substrate identification through covalent capture of phosphopeptides and mass spectrometry. (C) Stable isotope labeling of amino acids in culture (SILAC), is a versatile metabolic labeling proteomic technique that can be applied to study the effect of kinase inhibitors on neuronal growth by quantifying changes in proteome. Kino-beads can be employed for profiling the neuronal kinome in healthy and disease states. (D) Human iPSCs have been used to model neurological diseases associated with kinase genes.