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. 2021 Feb 10;9:585617. doi: 10.3389/fbioe.2021.585617

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Copyright © 2021 Guo, Hui, Zhang, Liu, Li and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Three cadmium biosensors were exposed to a range of metal ions at 5 μM. After induction with 5 μM various metal ions at 30^oC for 20 h, three kinds of reporter signals were determined. The bottom panels are the typical photographs of induced E. coli biosensors with 5 μM corresponding metal ions. (A) TOP10/pPcad-RFP. (B) TOP10/pPcad-RFP-GFP. (C) TOP10/pPcad-RFP-GFP-lacZα. Fluorescence intensity and enzymatic activity values were normalized using the absorbance at 600 nm. Data are representative of three independent experiments, and values are expressed as mean ± SD.