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. 2021 Feb 10;9:585617. doi: 10.3389/fbioe.2021.585617

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Copyright © 2021 Guo, Hui, Zhang, Liu, Li and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of reporter signal changes generated by three cadmium biosensors after exposure to different concentrations of Cd(II) and Hg(II). After induction with various concentrations of Cd(II) and Hg(II) at 30^oC for 20 h, three kinds of reporter signals were determined. (A) TOP10/pPcad-RFP. (B) TOP10/pPcad-RFP-GFP. (C) TOP10/pPcad-RFP-GFP-lacZα. Fluorescence intensity and enzymatic activity values were normalized using the absorbance at 600 nm. The data were obtained by subtracting the value of recombinant E. coli with no metal exposure from that of each group. Data are representative of three independent experiments, and values are expressed as mean ± SD.