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. 2021 Feb 23;10(4):e12072. doi: 10.1002/jev2.12072

FIGURE 5.

FIGURE 5

MiR‐155‐5p can be transferred from IR‐EVs to macrophages. [(a) Heat map showing the differential expressed miRNAs between S‐EVs and IR‐EVs. (b) The significantly high‐expressed miRNAs in IR‐EVs were verified by qPCR. (c) Confocal images showing the uptake of IR‐EVs by PMφ after coculture with DiI‐labelled EVs for 24 h. (d) qPCR was used to assess the expression of 3 IR‐EV–enriched miRNAs, including miR‐484, miR‐155‐5p and a novel miRNA (miR‐novel) in PMφ treated with PBS, S‐EVs or IR‐EVs for different times (0 h, 3 h, 24 h and 72 h). (e) Addition of increasing concentrations of IR‐EVs results in a dose‐dependent increase in miR‐155‐5p in treated macrophages. (f) A positive correlation between IR‐EV concentration and the expression of miR‐155‐5p in IR‐EV–treated macrophages. (g) The expression of pri‐mir155 in macrophages treated with gradient concentrations of IR‐EVs for 24 h. These data were representative results (n = 3) of three repetitions. *, P < 0.05;**, P < 0.01; ***, P < 0.001; ****, P<0.0001]