Skip to main content
. 2021 Feb 23;10(4):e12072. doi: 10.1002/jev2.12072

FIGURE 7.

FIGURE 7

IR‐EVs trigger systemic inflammation in the distant organs. [(a) Myocardial IR injury promoted the secretion of proinflammatory cytokines (IL1β and IL6) and chemokines (Cxcl1 and Cxcl2) in the blood. (b) Myocardial IR injury induced increased expressions of proinflammatory genes in the distant organs. (c) GW4869 treatment reduced the release of proinflammatory cytokines (IL1β and IL6) and chemokines (Cxcl1 and Cxcl2) in the circulation of IR‐injured mice. (d) GW4869 treatment decreased the expressions of proinflammatory genes induced by myocardial IR in the distant organs. (e) Bioluminescence imaging showed the distribution of IR‐EVs among the organs 24 h after intravenous injection of DiR‐labelled EVs. (f) The secretion of proinflammatory cytokines (IL1β and IL6) and chemokines (Cxcl1 and Cxcl2) in the blood 24 h after intravenous injection of 100 μL IR‐EVs (0.4 μg/μL) or PBS. (g) The expression of proinflammatory genes in the circulating leukocytes 24 h after intravenous injection of 100 μL IR‐EVs (0.4 μg/μL) or PBS. (h) The expression of proinflammatory genes in different organs 24 h after intravenous injection of 100 μL IR‐EVs (0.4 μg/μL) or PBS. These data were representative results (n = 3) of three repetitions. *, P < 0.05;**, P < 0.01; ***, P < 0.001; ****, P<0.0001]