Table 2.
RP-HPLC analysis of methylation level (%) in leaves of five tolerant (T) and five non-tolerant (NT) triticale lines after exposure to different Al3+ concentrations
| Line | C (0 ppm) | S (15 ppm) | S (20 ppm) | S (30 ppm) | Δ (S-C) (15 ppm) | Δ (S-C) (20 ppm) | Δ (S-C) (30 ppm) | |
|---|---|---|---|---|---|---|---|---|
| L438 | NT | 23.55 | 23.65 | 23.15 | 23.63 | 0.09 | − 0.40 | 0.08 |
| L444 | NT | 23.37 | 23.07 | 23.75 | 23.56 | − 0.29 | 0.38 | 0.19 |
| L455 | NT | 23.37 | 23.36 | 23.32 | 23.19 | − 0.01 | − 0.04 | − 0.17 |
| L461 | NT | 23.15 | 23.35 | 23.26 | 23.22 | 0.20 | 0.11 | 0.06 |
| L203 | NT | 23.90 | 23.83 | 24.02 | NA | − 0.07 | 0.12 | NA |
| ANOVA (NT lines) | 23.47 | 23.45 | 23.53 | 23.41 | − 0.02 | 0.03 | 0.04 | |
| Tukey’s grouping C, S, and Δ (S-C) | a | a | a | a | a | a | a | |
| L195 | T | 22.95 | 23.26 | 23.25 | NA | 0.31 | 0.30 | NA |
| L198 | T | 23.85 | 24.13 | 24.17 | 24.23 | 0.29 | 0.32 | 0.39 |
| L201 | T | 23.67 | 23.87 | 24.02 | 23.87 | 0.20 | 0.41 | 0.19 |
| L210 | T | 23.26 | 23.36 | 23.29 | 23.42 | 0.10 | 0.02 | 0.15 |
| L451 | T | 23.61 | 23.77 | 23.78 | 23.90 | 0.16 | 0.17 | 0.28 |
| ANOVA (T lines) | 23.47 | 23.67 | 23.72 | 23.85 | 0.21 | 0.24 | 0.25 | |
| Tukey’s grouping C, S, and Δ (S-C) | a | a | a | a | a | a | a | |
One-way analysis of variance (ANOVA) with Tukey’s contrasts (α = 0.05) of DNA methylation changes in three replicate experiments. Mean values (%) that do not share a letter are significantly different. C and S represent control and stressed conditions, respectively. Δ (S-C) reflects the methylation difference between C and S
NA, not analyzed