Table 4.

Changes in patterns of cytosine methylation in triticale roots under aluminum stress as determined by MSAP

Transitions		Effect	Percentage (%) of the bands representing particular effect
			NT						T
			L203	L438	L444	L455	L461	Mean^	L195	L198	L201	L210	L451	Mean^
IV → I		DM	1.14	2.05	2.05	1.15	1.15	1.74b	2.27	1.82	1.81	1.83	2.27	1.73b
IV → II		DM	1.60	1.60	1.60	1.84	1.84	1.69b	1.59	2.05	1.59	1.83	1.82	1.82b
II → I		DM	0.46	0.23	0.23	0.46	0.23	0.32a	0.23	0.45	0.45	0.46	0.23	0.41a
I → IV		DNM	0	0	0	0	0	0a	0.23	0.23	0	0	0	0.09a
III → III		LCMS	32.49	32.42	32.42	32.64	32.64	32.45d	32.27	32.27	32.20	32.49	32.27	32.38d
II → II		LCMS	48.74	48.40	48.40	48.51	48.51	48.45e	48.18	47.95	48.53	48.74	48.18	48.29e
I → I		LCMS	15.56	15.30	15.30	15.40	15.40	15.36c	15.23	15.23	15.42	15.56	15.23	15.28c
Total	Demethylation	DM	3.20	3.88	3.88	3.45	3.45	3.75	4.29	4.32	3.85	4.12	4.32	3.97
	Methylation de novo	DNM	0	0	0	0	0	0	0.23	0.23	0	0	0	0.09
	No change	LCMS	96.80	96.12	96.12	96.55	96.55	96.25	95.48	95.45	96.15	95.88	95.68	95.94

DM, demethylation event; DNM, de novo methylation event; LCMS, lack of change in methylation status; T, tolerant lines; NT, non-tolerant lines

LCMS % = 100 × LCMS / (LCMS + DNM + DM); DNM % = 100 × DNM / (LCMS + DNM + DM); DM % = 100 × DM / (LCMS + DNM + DM)

^{^}Means followed by the same letter in the same columns are not significantly different according to Tukey test (α = 0.05)