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. 2020 Dec 3;75(2):261–274. doi: 10.1007/s11418-020-01466-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Determination of the spiro-forming enzyme for the biosynthesis of spirotryprostatin A. a A biomimetic synthesis for producing spirotryprostatin scaffold. b NotB-catalyzed semipinacol-type rearrangement in the biosynthesis of notoamide C. c The enzymatic function of FqzB in fumiquinazoline biosynthesis. d The BGC of tryprostatin/fumitremorgin. e The BGC of fumiquinazolines. f In vitro enzymatic reaction of FqzB with tryprostatins A and B. g FqzB-catalyzed ortho-quinonemethide formation is the crucial step in the spiro-ring formation