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. 2020 Dec 3;75(2):261–274. doi: 10.1007/s11418-020-01466-x

Fig. 6.

Fig. 6

Activation of a silent gene cluster (pyn cluster) in A. niger. a The pyn gene cluster for the biosynthesis of pyranonigrin E1. b The experimental design for the overexpression of the transcription factor, pynR. c The culture of the pynR-expressed mutant (AnKW2) and the wild type (WT). d HPLC analysis of AnKW2 and WT. The chromatogram was monitored at λ = 280 nm. e RT-PCR analysis of the pyn cluster