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. 2021 Feb 23;11:4353. doi: 10.1038/s41598-021-83673-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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OPKA of rabbit antiserum. Sera from immunised rabbits were tested in an OPKA against GAS SF370 (M1T1 strain). (A) Representative line graph showing CFU recovered after incubation with serial dilutions of M1 (blue), T1 (green), and TeeVax1 (red) antiserum. Open symbols indicate pre-immune sera, solid symbols indicate immune sera. Serum dilution resulting in 50% killing is denoted by the point at which the curve crosses the dotted line which is used to calculate the opsonic index. (B) Opsonic index (n = 2) calculated from the serum dilution resulting in 50% killing. Each biological replicate is plotted (circles), with bar representing the mean. Hashed bars indicate pre-immune serum (Pre), solid bars indicate immune serum. Dashed line denotes the limit of detection.