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. 2021 Feb 23;11:4357. doi: 10.1038/s41598-021-82260-x

Figure 3.

Figure 3

Prolonged efficacy of SPD against TX100-induced cell death. Human keratinocytes (HaCaT) were first co-treated with SPD (100 mg/ml) and TX100 (0.2 mM) for 3 h. Cells were subsequently washed with PBS and incubated in a fresh medium for 24 h containing SPD and then analyzed for cell death by flow cytometry and confocal microscopy. (A,B) Flow cytometry histograms and quantification of %PI-positive cells. Data represent mean ± SEM (n = 6). *p < 0.05 compared to control (TX100-untreated). †p < 0.05 compared to TX100-treated group. (C,D) Confocal microscopy images and quantification of %PI-positive cells. Scale, 10 µm. Data represent mean ± SEM (n = 3–4). *p < 0.05 compared to control (TX100-untreated). †p < 0.05 compared to TX100-treated group.