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. 2021 Feb 23;11:4366. doi: 10.1038/s41598-021-83658-3

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Compounds 3A and 3D release from nanoparticles at different pH values. Release is reported as a percentage (%) release compared to total fluorescence at the time of sample preparation prior to t = 0 h. Compound 3A loaded nanoparticles show high initial dye release of 53% and 63% for pH 7.4 and 6.6, respectively, at t = 0 h. Release of compound 3A increased (t = 0 h to t = 4 h) to and plateaued (t = 4 h to t = 12 h) at 89% and 95% for pH 7.4 and 6.6, respectively. Compound 3D loaded nanoparticles released far less dye overall from t = 0 h to t = 12 h. Compound 3D showed no release at pH 7.4, 0.9% at t = 0 h to 1.0% at t = 12 h, and a modest release of 5.2% to 6.9% at pH 6.6 t = 0 h to t = 4 h increasing to only 15% by t = 12 h.