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. 2021 Feb 23;12:1240. doi: 10.1038/s41467-021-21509-5

Fig. 1. Capacity of LocSpiral to improve the interpretability of cryo-EM maps.

Fig. 1

A Top: sharpened maps of the TRP channel obtained by LocSpiral (left) and Relion postprocessing (right) methods. The threshold values are adjusted to provide similar densities in the core inner part of the protein. The red square in the figure shows a zoomed view of the protein inner core where both maps (LocSpiral and Relion) are superimposed. Relion map appears in red colour, while LocSpiral is in grey. Bottom: Fitted map densities (LocSpiral and Relion) with the corresponding atomic model (PDB ID: 6t9n) of two α-helices and one loop. The asterisks mark results obtained by LocSpiral approach. The residues marked with a red arrow were used to adjust the threshold values between maps. B Spliceosome maps at different orientations and similar threshold values obtained by LocSpiral and the postprocessing method of Relion.