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. 2021 Feb 10;11:619896. doi: 10.3389/fimmu.2020.619896

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Copyright © 2021 Hada-Neeman, Weiss-Ottolenghi, Wagner, Avram, Ashkenazy, Maor, Sklan, Shcherbakov, Pupko and Gershoni

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The construction of the “fth1-BC” phage-display vector. (A) fth1 vector: the fth1 vector has two cloning cassettes, in recombinant protein VIII (SfiI sites, purple) and in protein III (BstXI sites, orange). (B) The “fth1-BC” vector has been modified such that recombinant protein VIII and protein III genes are preceded by a barcode (BC) coding region. The barcode region codes for a total of ~1.6x10⁷ unique 12-bp DNA barcodes. In (C), the “fth1-BC” sequence and the Sanger sequencing-chromatogram illustrate the randomness of the barcode sequence.