Table 1.
Domain-Scan libraries.
| HIV-1 | HCV | |||||
|---|---|---|---|---|---|---|
| Antigen | p24 | gp160* | CORE | E2 | NS3 | NS5 |
| Length of peptide | 15aa | 50aa | 15aa | 20aa | 50aa | 50aa |
| No. of peptide | 41 | 83 | 27 | 75 | 81 | 41 |
| Shift | 5aa | 10aa | 5aa | 5aa | 10aa | 10aa |
| Displayed on | pVIII | pIII | pVIII | pVIII | pIII | pIII |
| Genotype | HIV-1 | HIV-1 | 1b | 1b | 1b | 1b |
For each antigen, we designed a library of overlapping peptides of various lengths and starting points. Peptides of 15 aa and 20 aa in length were cloned into pVIII, and peptides of 50 aa in length were cloned into pIII. Each of the HCV-NS3 and the HCV-E2 Domain-Scan libraries contain a few additional selected peptides ( Table S2 ). *9 domains were not used in the analysis due to technical problems (i.e., only 339 domains were used, see Table S2 ).