FIGURE 3.

Temporal expression of trehalose synthase (TPS), Rhopr-TRET, membrane-bound trehalase (m-Tre), and soluble trehalase (s-Tre) in the fat body and ovaries of R. prolixus under different nutritional conditions. Transcript expression of TPS, Rhopr-TRET, m-Tre, and s-Tre was quantified by RT-qPCR in the fat body (A–D, respectively) and ovaries (E–H, respectively) of adult females at varying days post-ecdysis (d PE), representing unfed conditions, and various days post-blood meal (d PBM), representing the fed condition. Results were analyzed by the ΔΔCt method. The y-axis represents the fold change in expression relative to 10 days PE (value ∼ 1). The results are shown as the mean ± SEM (n = 3–4, where each n represents a pool of tissues from 3 insects). Statistically significant differences were determined by a one-way ANOVA and a Tukey’s test as the post hoc test. Different letters indicate significant difference at P < 0.05.