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. 2021 Feb 10;12:624165. doi: 10.3389/fphys.2021.624165

FIGURE 5.

FIGURE 5

Participation of insulin and AKH signaling in Rhopr-TRET mRNA expression using in vivo assays. (A) Experimental scheme for (B–E). (B) Rhopr-TRET transcript expression after P_In injection; (C) TPS transcript expression after P_In injection; (D) Rhopr-TRET transcript expression after Rhopr-AKH injection. (E) TPS transcript expression after Rhopr-AKH injection. Rhopr-TRET and TPS transcript expression in ovaries of unfed adult females was tested by RT-qPCR. The y-axis represents the fold change in expression relative to control (saline, value ∼ 1). The results are shown as the mean ± SEM (n = 3–4, where each n represents a pool of tissues from 2 insects). Statistically significant differences were determined by the Student’s t-test.