FIGURE 7.

Transcript expression of Rhopr-AKHR and Rhopr-AKH in fat body, ovaries and CNS following ecdysis into adults. Rhopr-AKHR transcript expression in the fat body (A) and ovaries (B), and Rhopr-AKH transcript expression in the CNS (C) were assessed at various times post-ecdysis (PE) in adult females. The effects were measured by RT-qPCR. The y-axis represents the fold change in expression relative to 10 days PE (value ∼ 1). The results are shown as the mean ± SEM (n = 3–4, where each n represents a pool of tissues from 3 insects). The statistically significant differences were determined by a one-way ANOVA and a Tukey’s test as the post hoc test. Significance of P < 0.05 is denoted using letters to indicate bars that are significantly different from others. (D) Percent survival of dsRhopr-AKHR injected insects with respect to control (dsARG) over 3 days in the unfed condition (n = 15). Differences in the survival curve were analyzed using the Log-Rank test (*p < 0.001).