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. 2021 Feb 23;12:1248. doi: 10.1038/s41467-021-21451-6

Fig. 6. Ectopic expression of ENT3 confers taurine-conjugated bile acid-dependent amelioration of ER stress in cultured Slc29a3−/− HSPCs.

Fig. 6

a Flow cytometry quantification of the proportion of Slc29a3−/− HSPCs expressing ER stress markers GRP78 and GRP94 in the presence or absence of BAs. Slc29a3+/+(black), Slc29a3−/−(red), Slc29a3−/− + TUDCA (red open box), Slc29 a3−/− + TCA (red half-filled box), and Slc29a3−/− + Salubrinal (blue open box). Data represent mean ± SEM (n = 6 mice/group, *p < 0.05 by two-tailed t-test). b Taurine-conjugated BA (100 mM) promotes survival in Slc29a3+/+ (black) but not in Slc29a3−/− (red) bone marrow derived L T-HSCs that are treated with tunicamycin (10 mg/ml) or thapsigargin (10 mM). Data represent mean ± SEM (n = 6 mice/group, *p < 0.05 by ANOVA and Tukey’s post hoc test). c Taurine-conjugated BA (100 mM) promotes survival in Slc29a3+/+ (black) but not in Slc29a3−/− (red) fetal liver derived LT-HSCs that are treated with tunicamycin (10 mg/ml) or thapsigargin (10 mM). Data represent mean ± SEM (n = 6 mice/group, *p < 0.05 by ANOVA and Tukey’s post hoc test). d qPCR analysis of mRNA levels for ER stress marker genes GRP78 and GRP94 after treating Slc29a3+/+ or Slc29a3−/− LT-HSCs expressing GFP (GFP-OE) or ENT3 (ENT3-OE) with tunicamycin (2 mg/ml) or thapsigargin (2 mM) in the presence or absence of TUDCA. Slc29a3+/+GFP-OE (black), Slc29a3+/++ ENT3-OE (green), Slc29a3−/− + GFP-OE (red), and Slc29a3−/− + ENT3-OE (blue). Data represent mean ± SEM (n = 2 mice/group). e Flow cytometry-based quantification of proportion of HSPCs expressing ER stress markers GRP78 and GRP94 in Slc29a3+/+ or Slc29a3−/− cells after reintroducing ENT3 and preconditioning with taurine-conjugated BA for 48 h. Data represent mean ± SEM (n = 12 mice/group, *p < 0.05 by two-tailed t-test).