Table 4.
Betaine lipids identified in the polar lipidome of Chlorococcum amblystomatis by HILIC–HR–ESI–MS and MS/MS.
| Lipid species (C:N) | Calculated m/z | Observed m/z | Error (ppm) | Fatty acyl chains (C:N) | Formula |
|---|---|---|---|---|---|
| MGTS identified as [M+H]+ | |||||
| MGTS(14:0) | 446.3482 | 446.3479 | − 0.6 | 14:0 | C24H48O6N |
| MGTS(14:1) | 444.3325 | 444.3323 | − 0.5 | 14:1 | C24H46O6N |
| MGTS(16:0) | 474.3795 | 474.3793 | − 0.4 | 16:0 | C26H52O6N |
| MGTS(16:1) | 472.3638 | 472.3635 | − 0.7 | 16:1 | C26H50O6N |
| MGTS(16:2) | 470.3482 | 470.3485 | 0.7 | 16:2 | C26H48O6N |
| MGTS(16:3) | 468.3325 | 468.3335 | 2.1 | ** | C26H46O6N |
| MGTS(16:4) | 466.3169 | 466.3167 | − 0.4 | 16:4 | C26H44O6N |
| MGTS(18:0) | 502.4108 | 502.4115 | 1.5 | 18:0 | C28H56O6N |
| MGTS(18:1) | 500.3951 | 500.3948 | − 0.6 | 18:1 | C28H54O6N |
| MGTS(18:2) | 498.3795 | 498.3789 | − 1.1 | ** | C28H52O6N |
| MGTS(18:3) | 496.3638 | 496.3636 | − 0.4 | ** | C28H50O6N |
| MGTS(18:4) | 494.3482 | 494.3478 | − 0.7 | 18:4 | C28H48O6N |
| MGTS(18:5) | 492.3325 | 492.3318 | − 1.5 | 18:5 | C28H46O6N |
| MGTS(20:0) | 530.4421 | 530.4402 | − 3.5 | 20:0 | C30H60O6N |
| MGTS(20:4) | 522.3795 | 522.3798 | 0.6 | ** | C30H52O6N |
| MGTS(20:5) | 520.3638 | 520.3637 | − 0.2 | 20:5 | C30H50O6N |
| DGTS identified as [M+H]+ | |||||
| DGTS(28:1) | 654.5309 | 654.5325 | 2.4 | * | C38H72O7N |
| DGTS(30:0) | 684.5778 | 684.5759 | − 2.8 | 16:0–14:0 | C40H78O7N |
| DGTS(30:1) | 682.5622 | 682.5619 | − 0.4 | 16:1–14:0 | C40H76O7N |
| DGTS(30:2) | 680.5465 | 680.5444 | − 3.1 | 16:2–14:0 | C40H74O7N |
| DGTS(30:3) | 678.5309 | 678.5306 | − 0.4 | ** | C40H72O7N |
| DGTS(30:4) | 676.5152 | 676.5151 | − 0.1 | 16:4–16:0 | C40H70O7N |
| DGTS(30:5) | 674.4996 | 674.5002 | 0.9 | 16:4–14:1 | C40H68O7N |
| DGTS(32:0) | 712.6091 | 712.6067 | − 3.4 | 16:0–16:0 | C42H82O7N |
| DGTS(32:1) | 710.5935 | 710.5933 | − 0.3 | 16:1–16:0 | C42H80O7N |
| DGTS(32:2) | 708.5778 | 708.5772 | − 0.8 | 16:0–16:2 and 16:1–16:1 | C42H78O7N |
| DGTS(32:3) | 706.5622 | 706.5610 | − 1.7 | 16:3–16:0 | C42H76O7N |
| DGTS(32:4) | 704.5465 | 704.5464 | − 0.1 | 14:0–18:4, 16:4–16:0 and 16:3–16:1 | C42H74O7N |
| DGTS(32:5) | 702.5309 | 702.5306 | − 0.4 | ** | C42H72O7N |
| DGTS(32:6) | 700.5152 | 700.5152 | 0.0 | 16:4–16:2 and 16:3–16:3 | C42H70O7N |
| DGTS(32:7) | 698.4996 | 698.4993 | − 0.4 | 16:4–16:3 | C42H68O7N |
| DGTS(32:8) | 696.4839 | 696.4844 | 0.7 | 16:4–16:4 | C42H66O7N |
| DGTS(34:1) | 738.6248 | 738.6251 | 0.4 | 16:0–18:1 | C44H84O7N |
| DGTS(34:2) | 736.6091 | 736.6056 | − 4.8 | 16:1–18:1, 16:0–18:2 and 20:2–14:0 | C44H82O7N |
| DGTS(34:3) | 734.5935 | 734.5929 | − 0.8 | 16:2–18:1, 16:1–18:2 and 20:3–14:0 | C44H80O7N |
| DGTS(34:4) | 732.5778 | 732.5776 | − 0.3 | 18:4–16:0 and 18:3–16:1 | C44H78O7N |
| DGTS(34:5) | 730.5622 | 730.5616 | − 0.8 | 18:4–16:1, 18:1–16:4 and 18:2–16:3 | C44H76O7N |
| DGTS(34:5-OH) | 746.5571 | 746.5575 | 0.5 | 16:4-OH-18:1, 16:1-OH-18:4 and 16:0-OH-18:5 | C44H76O8N |
| DGTS(34:6) | 728.5465 | 728.5458 | − 1.0 | ** | C44H74O7N |
| DGTS(34:7) | 726.5309 | 726.5306 | − 0.4 | 16:4–18:3 and 16:3–18:4 | C44H72O7N |
| DGTS(34:7-OH) | 742.5258 | 742.5258 | 0.0 | 16:4-OH-18:3 and 16:3-OH-18:4 | C44H72O8N |
| DGTS(34:8) | 724.5152 | 724.5149 | − 0.5 | 16:4–18:4 | C44H70O7N |
| DGTS(36:10) | 748.5152 | 748.5140 | − 1.6 | 18:5–18:5 | C46H70O7N |
| DGTS(36:4) | 760.6091 | 760.6098 | 0.9 | 16:0–20:4, 18:3–18:1, 18:2–18:2, 20:3–16:1, 20:2–16:2, 20:1–16:3 and 20:0–16:4 | C46H82O7N |
| DGTS(36:5) | 758.5935 | 758.5933 | − 0.3 | 18:4–18:1, 20:5–16:0, 20:4–16:1, 20:3–16:2, 20:2–16:3, 20:1–16:4 and 16:1–20:4 | C46H80O7N |
| DGTS(36:6) | 756.5778 | 756.5775 | − 0.4 | 16:1–20:5, 20:3–16:3, 18:2–18:4 and 18:3–18:3 | C46H78O7N |
| DGTS(36:7) | 754.5622 | 754.5621 | − 0.1 | 18:4–18:3 | C46H76O7N |
| DGTS(36:8) | 752.5465 | 752.5462 | − 0.4 | 18:4–18:4 | C46H74O7N |
| DGTS(36:9) | 750.5309 | 750.5281 | − 3.7 | 18:5–18.4 | C46H72O7N |
| DGTS(38:10) | 776.5465 | 776.5464 | − 0.2 | 20:5–18:5 | C48H74O7N |
| DGTS(38:7) | 782.5935 | 782.5929 | − 0.8 | 20:5–18:2 | C48H80O7N |
| DGTS(38:8) | 780.5778 | 780.5743 | − 4.5 | 20:5–18:3 | C48H78O7N |
| DGTS(40:10) | 804.5778 | 804.5772 | − 0.7 | 20:5–20:5 | C50H78O7N |
| DGTS(40:9) | 806.5935 | 806.5905 | − 3.7 | 20:4–20:5 | C50H80O7N |
C number of carbon atoms, N number of double bonds.
*Identified based on the polar head fragmentation, calculated mass, and retention time.
**Identified according to the calculated mass and the retention time.