Table 5.
Glycolipids identified in the polar lipidome of Chlorococcum amblystomatis by HILIC–HR–ESI–MS and MS/MS.
| Lipid species (C:N) | Calculated m/z | Observed m/z | Error (ppm) | Fatty acyl chains (C:N) | Formula |
|---|---|---|---|---|---|
| MGMG identified as [M+NH4]+ | |||||
| MGMG(14:0) | 482.3329 | 482.3324 | − 1.1 | 14:0 | C23H48NO9 |
| MGMG(16:0) | 510.3642 | 510.3639 | − 0.6 | 16:0 | C25H52NO9 |
| MGMG(16:1) | 508.3486 | 508.3482 | − 0.7 | 16:1 | C25H50NO9 |
| MGMG(16:3) | 504.3173 | 504.3174 | 0.3 | ** | C25H46NO9 |
| MGMG(16:4) | 502.3016 | 502.3013 | − 0.6 | 16:4 | C25H44NO9 |
| MGMG(16:4-OH) | 518.2965 | 518.2963 | − 0.4 | 16:4-OH | C25H44NO9O |
| MGMG(18:1) | 536.3799 | 536.3793 | − 1.0 | 18:1 | C27H54NO9 |
| MGMG(18:3) | 532.3486 | 532.3485 | − 0.1 | 18:3 | C27H50NO9 |
| MGMG(18:4) | 530.3329 | 530.3330 | 0.2 | * | C27H48NO9 |
| MGMG(20:4) | 558.3642 | 558.3636 | − 1.1 | ** | C29H52NO9 |
| MGMG(20:5) | 556.3486 | 556.3482 | − 0.6 | ** | C29H50NO9 |
| MGDG identified as [M+NH4]+ | |||||
| MGDG(30:0) | 720.5625 | 720.5593 | − 4.4 | ** | C39H78NO10 |
| MGDG(30:1) | 718.5464 | 718.5466 | 0.3 | 16:1–14:0 | C39H76NO10 |
| MGDG(32:0) | 748.5939 | 748.5910 | − 3.8 | 16:0–16:0 | C41H82NO10 |
| MGDG(32:1) | 746.5777 | 746.5780 | 0.4 | 16:1–16:0 | C41H80NO10 |
| MGDG(32:2) | 744.5626 | 744.5624 | − 0.3 | 16:1–16:1, 14:0–18:2 and 16:0–16:2 | C41H78NO10 |
| MGDG(32:3) | 742.5464 | 742.5467 | 0.4 | ** | C41H76NO10 |
| MGDG(32:4) | 740.5307 | 740.5305 | − 0.3 | ** | C41H74NO10 |
| MGDG(32:5) | 738.5156 | 738.5158 | 0.2 | ** | C41H72NO10 |
| MGDG(32:6) | 736.4994 | 736.4999 | 0.7 | ** | C41H70NO10 |
| MGDG(32:7) | 734.4843 | 734.4835 | − 1.1 | ** | C41H68NO10 |
| MGDG(32:8) | 732.4687 | 732.4686 | − 0.1 | 16:4–16:4 | C41H66NO10 |
| MGDG(34:1) | 774.6090 | 774.6073 | − 2.2 | ** | C43H84NO10 |
| MGDG(34:2) | 772.5933 | 772.5926 | − 0.9 | 18:2–16:0 | C43H82NO10 |
| MGDG(34:3) | 770.5782 | 770.5782 | 0.0 | 18:3–16:0 and 18:2–16:1 | C43H80NO10 |
| MGDG(34:4) | 768.5626 | 768.5645 | 2.5 | 16:2–18:2, 16:1–18:3, 16:0–18:4, 16:3–18:1 and 16:4–18:0 | C43H78NO10 |
| MGDG(34:5) | 766.5469 | 766.5474 | 0.6 | * | C43H76NO10 |
| MGDG(34:7) | 762.5156 | 762.5158 | 0.2 | 16:4–18:3 and 18:4–16:3 | C43H72NO10 |
| MGDG(34:8) | 760.5000 | 760.4999 | − 0.1 | ** | C43H70NO10 |
| MGDG(36:3) | 798.6095 | 798.6055 | − 5.0 | ** | C45H84NO10 |
| MGDG(36:4) | 796.5933 | 796.5892 | − 5.1 | ** | C45H82NO10 |
| MGDG(36:5) | 794.5782 | 794.5778 | − 0.5 | 18:3–18:2, 18:1–18:4, 20:5–16:0, 16:4–20:2 and 16:1–20:4 | C45H80NO10 |
| MGDG(36:6) | 792.5625 | 792.5623 | − 0.3 | 18:3–18:3, 16:1–20:5 and 16:4–20:2 | C45H78NO10 |
| MGDG(36:7) | 790.5469 | 790.5470 | 0.1 | ** | C45H76NO10 |
| MGDG(36:8) | 788.5313 | 788.5339 | 3.3 | ** | C45H74NO10 |
| MGDG(38:6) | 820.5939 | 820.5931 | − 1.0 | 18:1–20:5, 18:2–20:4 and 18:3–20:3 | C47H82NO10 |
| MGDG(38:7) | 818.5782 | 818.5777 | − 0.6 | 20:5–18:2 and 18:3–20:4 | C47H80NO10 |
| MGDG(38:8) | 816.5626 | 816.5614 | − 1.4 | ** | C47H78NO10 |
| MGDG(40:10) | 840.5626 | 840.5622 | − 0.4 | 20:5–20:5 | C49H78NO10 |
| MGDG(40:8) | 844.5939 | 844.5916 | − 2.7 | 20:4–20:4 | C49H82NO10 |
| MGDG(40:7) | 846.6095 | 846.6097 | 0.2 | ** | C49H84NO10 |
| DGMG identified as [M+NH4]+ | |||||
| DGMG(14:0) | 644.3857 | 644.3848 | − 1.4 | ** | C29H58NO14 |
| DGMG(16:0) | 672.4170 | 672.4172 | 0.3 | 16:0 | C31H62NO14 |
| DGMG(16:1) | 670.4014 | 670.4012 | − 0.3 | 16:1 | C31H60NO14 |
| DGMG(16:2) | 668.3857 | 668.3854 | − 0.5 | ** | C31H58NO14 |
| DGMG(16:3) | 666.3701 | 666.3699 | − 0.3 | ** | C31H56NO14 |
| DGMG(16:4) | 664.3544 | 664.3541 | − 0.5 | ** | C31H54NO14 |
| DGMG(18:1) | 698.4327 | 698.4327 | 0.0 | 18:1 | C33H64NO14 |
| DGMG(18:2) | 696.4170 | 696.4149 | − 3.0 | ** | C33H62NO14 |
| DGMG(18:3) | 694.4014 | 694.4013 | − 0.1 | 18:3 | C33H60NO14 |
| DGMG(18:4) | 692.3857 | 692.3856 | − 0.2 | ** | C33H58NO14 |
| DGMG(20:5) | 718.4014 | 718.4009 | − 0.7 | ** | C35H60NO14 |
| DGDG identified as [M+NH4]+ | |||||
| DGDG(30:0) | 882.6154 | 882.6111 | − 4.9 | ** | C45H88O15N |
| DGDG(30:1) | 880.5997 | 880.5990 | − 0.8 | 16:1–14:0 | C45H86O15N |
| DGDG(32:1) | 908.6310 | 908.6306 | − 0.4 | 16:0–16:1 | C47H90O15N |
| DGDG(32:2) | 906.6154 | 906.6151 | − 0.3 | 16:0–16:2 and 16:1–16:1 | C47H88O15N |
| DGDG(32:3) | 904.5997 | 904.6011 | 1.5 | 18:3–14:0, 16:0–16:3 and 16:1–16:2 | C47H86O15N |
| DGDG(32:4) | 902.5841 | 902.5846 | 0.6 | 18:3–14:1, 16:0–16:4 and 14:0–18:4 | C47H84O15N |
| DGDG(32:5) | 900.5684 | 900.5678 | − 0.7 | 16:1–16:4 | C47H82O15N |
| DGDG(32:6) | 898.5528 | 898.5512 | − 1.8 | ** | C47H80O15N |
| DGDG(34:1) | 936.6623 | 936.6615 | − 0.9 | 18:1–16:0 | C49H94O15N |
| DGDG(34:2) | 934.6467 | 934.6443 | − 2.6 | 18:1–16:1 and 18:2–16:0 | C49H92O15N |
| DGDG(34:3) | 932.6310 | 932.6309 | − 0.1 | 16:0–18:3 and 18:1–16:2 | C49H90O15N |
| DGDG(34:4) | 930.6154 | 930.6143 | − 1.2 | 18:1–16:3, 18:2–16:2, 18:3–16:1, 18:4–16:0 and 14:0–20:4 | C49H88O15N |
| DGDG(34:5) | 928.5997 | 928.5991 | − 0.6 | 18:3–16:2, 18:2–16:3, 18:1–16:4, 16:1–18:4 and 14:0–20:5 | C49H86O15N |
| DGDG(34:6) | 926.5841 | 926.5820 | − 2.3 | ** | C49H84O15N |
| DGDG(34:7) | 924.5684 | 924.5684 | 0.0 | 18:3–16:4 and 18:4–16:3 | C49H82O15N |
| DGDG(34:8) | 922.5528 | 922.5501 | − 2.9 | ** | C49H80O15N |
| DGDG(36:2) | 962.6780 | 962.6751 | − 3.0 | ** | C51H96O15N |
| DGDG(36:3) | 960.6623 | 960.6606 | − 1.8 | 18:3–18:0 and 18:1–18:2 | C51H94O15N |
| DGDG(36:4) | 958.6467 | 958.6433 | − 3.5 | ** | C51H92O15N |
| DGDG(36:5) | 956.6310 | 956.6301 | − 0.9 | 20:5–16:0, 18:1–18:4 and 20:1–16:4 | C51H90O15N |
| DGDG(36:6) | 954.6154 | 954.6152 | − 0.2 | 18:3–18:3, 20:5–16:1 and 18:4–18:2 | C51H88O15N |
| DGDG(36:7) | 952.5997 | 952.5999 | 0.2 | 18:3–18:4 | C51H86O15N |
| DGDG(36:8) | 950.5841 | 950.5835 | − 0.6 | 18:4–18:4 | C51H84O15N |
| DGDG(38:6) | 982.6467 | 982.6421 | − 4.7 | ** | C53H92O15N |
| DGDG(38:7) | 980.6310 | 980.6312 | 0.2 | 18:2–20:5 | C53H90O15N |
| DGDG(40:10) | 1002.6154 | 1002.6148 | − 0.6 | 20:5–20:5 | C55H88O15N |
| DGDG(40:9) | 1004.6310 | 1004.6323 | 1.3 | ** | C55H90O15N |
| SQDG identified as [M−H]− | |||||
| SQDG(28:0) | 737.4510 | 737.4492 | − 2.4 | ** | C37H69O12S |
| SQDG(30:0) | 765.4823 | 765.4818 | − 0.6 | 14:0–16:0 | C39H73O12S |
| SQDG(30:1) | 763.4666 | 763.4667 | 0.1 | 14:0–16:1 | C39H71O12S |
| SQDG(32:0) | 793.5136 | 793.5135 | − 0.1 | ** | C41H77O12S |
| SQDG(32:1) | 791.4979 | 791.4982 | 0.3 | 16:0–16:1 | C41H75O12S |
| SQDG(32:2) | 789.4823 | 789.4821 | − 0.2 | 16:1–16:1 and 16:0–16:2 | C41H73O12S |
| SQDG(32:3) | 787.4666 | 787.4668 | 0.2 | 16:1–16:2, 16:0–16:3 and 18:1–14:1 | C41H71O12S |
| SQDG(32:4) | 785.4510 | 785.4517 | 0.9 | 16:0–16:4, 16:1–16:3 and 16:2–16:2 | C41H69O12S |
| SQDG(34:0) | 821.5449 | 821.5446 | − 0.3 | 16:0–18:0 | C43H81O12S |
| SQDG(34:1) | 819.5292 | 819.5296 | 0.5 | 16:0–18:1 | C43H79O12S |
| SQDG(34:3) | 815.4979 | 815.4982 | 0.3 | 16:0–18:3 and 16:2–18:1 | C43H75O12S |
| SQDG(34:4) | 813.4823 | 813.4829 | 0.8 | 16:0–18:4, 16:1–18:3, 16:2–18:2, 18:1–16:3 and 14:0–20:4 | C43H73O12S |
| SQDG(34:5) | 811.4666 | 811.4667 | 0.1 | 16:0–18:5, 16:1–18:4, 16:2–18:3, 16:3–18:2 and 16:4–18:1 | C43H71O12S |
| SQDG(36:0) | 849.5762 | 849.5761 | − 0.1 | 16:0–20:0 | C45H85O12S |
| SQDG(36:3) | 843.5292 | 843.5287 | − 0.6 | ** | C45H79O12S |
| SQDG(36:4) | 841.5136 | 841.5135 | − 0.1 | 16:0–20:4 | C45H77O12S |
| SQDG(36:5) | 839.4979 | 839.4987 | 0.9 | ** | C45H75O12S |
| SQDG(36:6) | 837.4823 | 837.4823 | 0.0 | 16:1–20:5, 20:4–16:2, 18:2–18:4 and 18:3–18:3 | C45H73O12S |
| SQDG(36:7) | 835.4666 | 835.4676 | 1.2 | 18:3–18:4, 20:4–16:3 and 20:5–16:2 | C45H71O12S |
| SQDG(34:3-OH) | 831.4928 | 831.4930 | 0.2 | – | C43H75O13S |
| SQDG(34:4-OH) | 829.4772 | 829.4752 | − 2.4 | – | C43H73O13S |
| SQMG identified as [M−H]− | |||||
| SQMG(16:0) | 555.2839 | 555.2838 | − 0.2 | 16:0 | C25H47O11S |
C carbons, N number of double bonds.
*Identified based on the polar head fragment, calculated mass and retention time.
**Identified based on calculated mass and retention time.