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. 2021 Feb 23;12:1251. doi: 10.1038/s41467-021-21512-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a The distribution of ciliated cells is similar in the epithelium of control Adgrd^+/− and Adgrd1^−/− oviducts. Oviductal sections from adult females in diestrous were stained with an antibody against acetylated tubulin to mark cilia (green), and nuclei counterstained with DAPI (blue, right panel). b, b’ Ciliated cells analysed by scanning electron microscopy did not differ in mutant Adgrd1^−/− oviducts compared to wild-type controls. c Transmission electron microscopy images of Adgrd1^−/− cilia showed the usual 9 + 2 organisation of microtubules. d Polystyrene beads placed on oviductal epithelium explants moved at equivalent speeds in an abovarial direction in both control and Adgrd1^−/− epithelial tissues. Individual data points are bead velocity quantified with Image J manual tracking for a minimum of 15 s. Bars represent the mean ± SEM, measurements were performed on 3 Adgrd^+/+ and 3 Adgrd1^−/− ampullae; an unpaired t-test analysis showed no significant (ns) difference between the groups. e Image shows 3D projection of oviducts from 17-day-old mice stained with a phalloidin-Texas Red conjugate, demonstrating no overt difference in muscle structure and organisation between wild type and mutant. f and f’ Representative examples of sections of the isthmus stained with anti-smooth muscle alpha actin (magenta) and counterstained with DAPI (Cyan). g The thickness of the myosalpinx is similar in controls (hues of grey) and Adgrd1^−/− (hues of red) in the different oviductal regions. Bars represent the mean ± SEM, measurements were performed on 3 Adgrd^+/− and 3 Adgrd1^−/− oviducts; a minimum of two sections per mouse were analysed. A two-way ANOVA analysis found that the genotype has no effect (ns) while the difference between the regions of the oviduct is extremely significant (p < 0.0001). a, b, b’, c, e, f, and f’ are representative examples of three independent experiments.