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. 2021 Feb 10;15:629356. doi: 10.3389/fncel.2021.629356

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Copyright © 2021 Yan, Shi, Wang, Si, Liu and Du.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Primary culture of BMSCs and identification. (A) The morphology of BMSCs from passage 1 to passage 3 was observed under the microscope. (B) The expression rates of CD90, CD44, CD45, and CD34 were detected by flow cytometry. (C) The osteogenic differentiation capability of BMSCs was assessed by alizarin red staining. (D) The adipogenic differentiation capability of BMSCs was assessed by Oil Red-O-staining. The results displayed were obtained from at least three independent experiments.