Figure 1.

Scanning electron microscopy of neutrophils challenged or not with P. brasiliensis (50:1 ratio), showing different patterns of neutrophil extracellular traps (NETs) release (C, D). All cultures were incubated during 2 h at 37°C at 5% CO2, with or not PMA+DNase (control cultures). (A) normal neutrophils; (B) neutrophils treated with PMA plus DNase, showing absence of NETs; (C) neutrophils challenged with Pb18 showing looser, dispersed and bigger aspect of NETs; (D) neutrophils challenged with Pb265 showing denser, more condensed and compacter aspect of NETs. The images are reprentative of 10 individuals tested.