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. 2021 Feb 10;10:592022. doi: 10.3389/fcimb.2020.592022

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Copyright © 2021 Zonta, Dezen, Della Coletta, Yu, Carvalho, Santos, Deprá, Kratofil, Willson, Zbytnuik, Kubes, Ximenes and Dias-Melicio

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Confocal microscopy of neutrophils challenged with P. brasiliensis - Pb265 (50:1 ratio), showing the pattern of neutrophil extracellular traps (NETs) release. Cocultures were stained with DAPI (A), labeled with anti-elastase antibody followed by FITC-conjugated secondary antibody (B), and anti-histone H1 secondary antibody followed by Texas Red (C). In the last frame, the overlapping images showing the three components of NETs (D). (Bar size 50 μm). The images are reprentative of 10 individuals tested.