Skip to main content
. 2021 Feb 10;12:631889. doi: 10.3389/fmicb.2021.631889

FIGURE 1.

FIGURE 1

Infection of honey bee pupal tissues with DWV. (A) Image of the cultured head tissue from pale eyes-pupa. (B) Abdomen or head of the single pupa was sagittally halved and one tissue was infected by DWV (+) whereas the other was left untreated (−). DWV infection was tested through western blotting using anti-RdRP antibody. RdRP precursor with 3C-protease (90 kDa) and mature RdRP (53 kDa) bands are indicated with black and white arrowheads, respectively. The molecular weight (kDa) of the protein marker is at the left. (C) Synthesis of RdRP in the pupal head tissue was tested at 6, 12, and 24 h after DWV infection (hpi). The star represents a protein that non-specifically cross-reacted with anti-RdRP antibody. The presence of this band indicates that an equal amount of protein was loaded in each lane. (D) Band intensity of the RdRP precursor was compared between three time points using the one-tailed Dunnett test and P-values between 6 and 12 or 24 h are <1.3 × 10–6 and <7.2 × 10–6, respectively (**). Mean values ± SD (error bars) are shown (n = 6). *This represents statistical significance.